As haidee said, physical traits are linked to the Ascendant.
The chart through Astrotheme is not very clear because it shows sign division in preference to house cusps.
Is the chart tropical or sidereal, geo- or helio- centric??
Virgo is the sign of 'being average and ordinary'. It can represents things in miniature rather than major.
From the medical/health astrology perspective, using Western tropical astrology:
When a planet is in the 1st house with adverse aspects, it can denote a physical issue from birth. Such would raise an eyebrow when Moon is involved; childhood and physical issues it can bring?
Looking at Moon's aspects:
Moon rules all fluids in the body. It is on the Ebertin degree that is associated with spinal issues.
It makes an adverse aspect to natal Mercury, ruler Ascendant, in Scorpio. Scorpio rules generative cells.
Moon is exact quincunx/inconjunct Saturn in Aquarius (on 6th house cusp?) Saturn is associated with the skeletal bone structure.
Aquarius with lower leg (knee to ankle), but also with the vertebrae (spine).
Where physical issues are dominant, Saturn would seem more apparent as traditional ruler Aquarius than Uranus, its modern ruler.
Moon is trine the generational Uranus-Neptune in Capricorn. The conjunction says much about lack of structural grounding.
There is a Sun-Pluto square Saturn. Sun is ruler of the heart but through Leo also has associations with the spinal column.
Jupiter-Saturn have shown conditions regarding excessive/stinted growth. There is a Jupiter trine Saturn in the chart.
First thoughts would bë:
Conditions regarding growth through bone structure of the spine, that could result in restriction of agility/movement (3rd house influence).
Second thoughts would run towards difficulties in the autonomous nervous system; a form of paralysis that restricts agility of movement, yet is not necessarily influential upon the 3rd house mental faculties .(You did not say if there were issues regarding mentality).
Third thoughts might tally with a Virgo-Saturn influenced skin disorder.... psoriasis, scaly skin, eczema. etc.
Fourth thoughts of an entirely different nature would be of gender issues (M.C. in Gemini with Asc. Virgo can show 'wrong body' ideas). I read somewhere that Mars-Pluto ( cell reproduction) aspects can refer to the function of 'feeling' that one is going from one gender to another. Mars is conjunct North node.
Then come all the further thoughts, including an inferiority complex?
For the sake of those of us always learning, are you willing to share what your brother's physical issue is?